Demulsification examples11/1/2023 ![]() The method of claim 32, wherein the fermentation broth has a biomass density of at least 100 g/1.ģ4. The method of claim 16, wherein the suspension is provided as a fermentation broth.ģ3. The method of claim 16, wherein steps (c) to (f) are carried out without prior lysing of the cells of the biomass.ģ2. The method of claim 16, wherein cells are lysed enzymatically, mechanically, chemically and/or physically.ģ1. The method of claim 16, wherein cells are lysed using either no organic solvent or organic solvent in an amount of less than 0.1 g/l fermentation broth.ģ0. The method of claim 16, wherein cells are lysed using either no salt or salt at less than 0.1 g/l fermentation broth.Ģ9. The method of claim 16, wherein the suspension as provided in step (a) has a total dry matter content of 20 to 60 wt.-%.Ģ8. The method of claim 16, wherein concentration of the suspension in step (b) is carried out by evaporation of water at a temperature not higher than 100° C.Ģ7. The method of claim 16, wherein separating the lipids containing light phase from the water and cell debris containing heavy phase according to step (f) is carried out by centrifugation or filtration.Ģ6. The method of claim 16, wherein the continuous reactor of step (e) is a column reactor, a tube or a plug-flow reactor.Ģ5. The method of claim 16, wherein the suspension leaving the continuous reactor has, or adjusted to have, a pH value of between 5.5 to 8.5 before the separation of the lipids containing light phase from the water and cell debris containing heavy phase is carried out.Ģ4. The method of claim 16, wherein the base as added in step (d) is sodium hydroxide provided as an aqueous solution.Ģ3. The method of claim 16, wherein the base as added in step (d) is a hydroxide and/or carbonate and/or a bicarbonate.Ī) the hydroxide is selected from the group consisting of: sodium hydroxide lithium hydroxide potassium hydroxide and/or calcium hydroxide b) the carbonate is selected from the group consisting of: sodium carbonate potassium carbonate and/or magnesium carbonate and/or c) the bicarbonate is selected from the group consisting of: lithium bicarbonate sodium bicarbonate and potassium bicarbonate.Ģ2. The method of claim 16, wherein the suspension is mixed with the base equivalents in a static mixer, and wherein the static mixer forms part of the continuous reactor or is located just before the continuous reactor.Ģ0. The method of claim 16, wherein in step d), base equivalents are added to the suspension to adjust the pH of the suspension to a value of 8 to 11.5.ġ9. The method of claim 16, wherein, in step d), 12 to 17 moles of base equivalent are added to 10 kg of total dry matter in the suspension and, in step e), the hydrodynamic residence time is 2 to 24 hours at a temperature of 70 to 90° C.ġ8. f) separating the lipids containing light phase from the water and cell debris containing heavy phase.ġ7. A method of isolating a polyunsaturated fatty acids (PUFAs) containing lipid from a biomass, comprising the following steps:Ī) providing a suspension of a biomass comprising cells which contain a PUFAs containing lipid b) optionally lysing the cells of the biomass c) concentrating the suspension to a total dry matter content (TDM) of 20 to 60 wt.-%, if the suspension has a lower TDM d) adding a total of 7.5 to 25 moles of base equivalent to 10 kg of total dry matter as contained in the suspension e) feeding a continuous reactor with the suspension at a hydrodynamic residence time of 2 to 36 hours at a temperature of 20° C. Usually, the amount of the demulsifier composition may be 20-1000 μg, preferably 100-500 μg, per gram of the acidified oil-c.16. Since acidified oil is an extremely inferior crude oil, the amount of the demulsifier composition is generally relatively large. In the raw oil containing acidified oil, based on the total weight of the raw oil containing acidified oil, the content of acidified oil may be 10-100% by weight. The feed oil containing acidified oil may be a mixed feed oil blended with acidized oil and conventional crude oil, or may be a single acidized oil. The present invention also provides a method for demulsifying acidified oil, the method comprising: using the demulsifier composition provided by the present invention to perform demulsification treatment on raw oil containing acidified oil. The preparation method of the demulsifier composition provided by the present invention is relatively simple, and only needs to mix each component uniformly.
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